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rm(list = ls())
library(Seurat)
library(dplyr)
library(patchwork)
library(magrittr)
# Load the LC dataset
Loco.data <- Read10X(data.dir = "~/data/Locus_Coruleus2/count/")
# Initialize the Seurat object with the raw (non-normalized data).
Loco <- CreateSeuratObject(counts = Loco.data, project = "Locus_Coruleus", min.cells = 3, min.features = 200)
# The [[ operator can add columns to object metadata. This is a great place to stash QC stats
Loco[["percent.mt"]] <- PercentageFeatureSet(Loco, pattern = "^mt-")
# Visualize QC metrics as a violin plot
VlnPlot(Loco, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
# FeatureScatter is typically used to visualize feature-feature relationships, but can be used
# for anything calculated by the object, i.e. columns in object metadata, PC scores etc.
plot1 <- FeatureScatter(Loco, feature1 = "nCount_RNA", feature2 = "percent.mt")
plot2 <- FeatureScatter(Loco, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
plot1 + plot2
Loco <- subset(Loco, subset = nFeature_RNA > 200 & percent.mt < 20)
# normalize and run dimensionality reduction on control dataset
Loco <- SCTransform(Loco, return.only.var.genes = FALSE, verbose = FALSE, variable.features.n = 10000) %>%
RunPCA(npcs = 100, verbose = FALSE) %>%
RunUMAP(dims = 1:20, verbose = FALSE) %>%
FindNeighbors(reduction = "pca", dims = 1:100, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)
DimHeatmap(Loco, dims = 1:20, cells = 500, balanced = TRUE)
ElbowPlot(Loco, ndims = 30)
DimPlot(Loco, reduction = "umap",label = T)
Loco.markers <- FindAllMarkers(Loco, only.pos = TRUE, test.use = "wilcox", min.pct = 0.1, logfc.threshold = 0.25)Calculating cluster 0
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|+++++++++++++++++++++++++++ | 54% ~01s
|++++++++++++++++++++++++++++ | 55% ~01s
|++++++++++++++++++++++++++++ | 56% ~01s
|+++++++++++++++++++++++++++++ | 57% ~01s
|+++++++++++++++++++++++++++++ | 58% ~01s
|++++++++++++++++++++++++++++++ | 59% ~01s
|++++++++++++++++++++++++++++++ | 60% ~01s
|+++++++++++++++++++++++++++++++ | 61% ~01s
|+++++++++++++++++++++++++++++++ | 62% ~01s
|++++++++++++++++++++++++++++++++ | 63% ~01s
|++++++++++++++++++++++++++++++++ | 64% ~01s
|+++++++++++++++++++++++++++++++++ | 65% ~01s
|+++++++++++++++++++++++++++++++++ | 66% ~01s
|++++++++++++++++++++++++++++++++++ | 67% ~01s
|++++++++++++++++++++++++++++++++++ | 68% ~01s
|+++++++++++++++++++++++++++++++++++ | 69% ~01s
|+++++++++++++++++++++++++++++++++++ | 70% ~01s
|++++++++++++++++++++++++++++++++++++ | 71% ~01s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=02s Calculating cluster 7
| | 0 % ~calculating
|+ | 1 % ~01s
|++ | 2 % ~01s
|++ | 3 % ~01s
|+++ | 4 % ~01s
|+++ | 5 % ~01s
|++++ | 6 % ~01s
|++++ | 7 % ~01s
|+++++ | 8 % ~01s
|+++++ | 9 % ~01s
|++++++ | 10% ~01s
|++++++ | 11% ~01s
|+++++++ | 12% ~01s
|+++++++ | 14% ~01s
|++++++++ | 15% ~01s
|++++++++ | 16% ~01s
|+++++++++ | 17% ~01s
|+++++++++ | 18% ~01s
|++++++++++ | 19% ~01s
|++++++++++ | 20% ~01s
|+++++++++++ | 21% ~01s
|+++++++++++ | 22% ~01s
|++++++++++++ | 23% ~01s
|++++++++++++ | 24% ~01s
|+++++++++++++ | 25% ~01s
|++++++++++++++ | 26% ~01s
|++++++++++++++ | 27% ~01s
|+++++++++++++++ | 28% ~01s
|+++++++++++++++ | 29% ~01s
|++++++++++++++++ | 30% ~00s
|++++++++++++++++ | 31% ~00s
|+++++++++++++++++ | 32% ~00s
|+++++++++++++++++ | 33% ~00s
|++++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 35% ~00s
|+++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 38% ~00s
|++++++++++++++++++++ | 39% ~00s
|++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|+++++++++++++++++++++ | 42% ~00s
|++++++++++++++++++++++ | 43% ~00s
|++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|+++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|+++++++++++++++++++++++++ | 50% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|++++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|+++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|++++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|+++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|+++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=01s Calculating cluster 8
| | 0 % ~calculating
|+ | 1 % ~02s
|++ | 2 % ~02s
|++ | 3 % ~02s
|+++ | 4 % ~02s
|+++ | 5 % ~02s
|++++ | 6 % ~02s
|++++ | 7 % ~02s
|+++++ | 8 % ~02s
|+++++ | 9 % ~02s
|++++++ | 10% ~02s
|++++++ | 11% ~02s
|+++++++ | 12% ~02s
|+++++++ | 13% ~02s
|++++++++ | 14% ~02s
|++++++++ | 15% ~02s
|+++++++++ | 16% ~02s
|+++++++++ | 18% ~02s
|++++++++++ | 19% ~02s
|++++++++++ | 20% ~02s
|+++++++++++ | 21% ~02s
|+++++++++++ | 22% ~02s
|++++++++++++ | 23% ~02s
|++++++++++++ | 24% ~02s
|+++++++++++++ | 25% ~02s
|+++++++++++++ | 26% ~02s
|++++++++++++++ | 27% ~02s
|++++++++++++++ | 28% ~02s
|+++++++++++++++ | 29% ~02s
|+++++++++++++++ | 30% ~02s
|++++++++++++++++ | 31% ~02s
|++++++++++++++++ | 32% ~02s
|+++++++++++++++++ | 33% ~02s
|++++++++++++++++++ | 34% ~02s
|++++++++++++++++++ | 35% ~02s
|+++++++++++++++++++ | 36% ~01s
|+++++++++++++++++++ | 37% ~01s
|++++++++++++++++++++ | 38% ~01s
|++++++++++++++++++++ | 39% ~01s
|+++++++++++++++++++++ | 40% ~01s
|+++++++++++++++++++++ | 41% ~01s
|++++++++++++++++++++++ | 42% ~01s
|++++++++++++++++++++++ | 43% ~01s
|+++++++++++++++++++++++ | 44% ~01s
|+++++++++++++++++++++++ | 45% ~01s
|++++++++++++++++++++++++ | 46% ~01s
|++++++++++++++++++++++++ | 47% ~01s
|+++++++++++++++++++++++++ | 48% ~01s
|+++++++++++++++++++++++++ | 49% ~01s
|++++++++++++++++++++++++++ | 51% ~01s
|++++++++++++++++++++++++++ | 52% ~01s
|+++++++++++++++++++++++++++ | 53% ~01s
|+++++++++++++++++++++++++++ | 54% ~01s
|++++++++++++++++++++++++++++ | 55% ~01s
|++++++++++++++++++++++++++++ | 56% ~01s
|+++++++++++++++++++++++++++++ | 57% ~01s
|+++++++++++++++++++++++++++++ | 58% ~01s
|++++++++++++++++++++++++++++++ | 59% ~01s
|++++++++++++++++++++++++++++++ | 60% ~01s
|+++++++++++++++++++++++++++++++ | 61% ~01s
|+++++++++++++++++++++++++++++++ | 62% ~01s
|++++++++++++++++++++++++++++++++ | 63% ~01s
|++++++++++++++++++++++++++++++++ | 64% ~01s
|+++++++++++++++++++++++++++++++++ | 65% ~01s
|+++++++++++++++++++++++++++++++++ | 66% ~01s
|++++++++++++++++++++++++++++++++++ | 67% ~01s
|+++++++++++++++++++++++++++++++++++ | 68% ~01s
|+++++++++++++++++++++++++++++++++++ | 69% ~01s
|++++++++++++++++++++++++++++++++++++ | 70% ~01s
|++++++++++++++++++++++++++++++++++++ | 71% ~01s
|+++++++++++++++++++++++++++++++++++++ | 72% ~01s
|+++++++++++++++++++++++++++++++++++++ | 73% ~01s
|++++++++++++++++++++++++++++++++++++++ | 74% ~01s
|++++++++++++++++++++++++++++++++++++++ | 75% ~01s
|+++++++++++++++++++++++++++++++++++++++ | 76% ~01s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~01s
|++++++++++++++++++++++++++++++++++++++++ | 78% ~01s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=02s Calculating cluster 9
| | 0 % ~calculating
|+ | 1 % ~01s
|++ | 2 % ~01s
|++ | 4 % ~01s
|+++ | 5 % ~01s
|+++ | 6 % ~01s
|++++ | 7 % ~01s
|+++++ | 8 % ~01s
|+++++ | 10% ~01s
|++++++ | 11% ~01s
|++++++ | 12% ~01s
|+++++++ | 13% ~01s
|++++++++ | 14% ~01s
|++++++++ | 15% ~01s
|+++++++++ | 17% ~01s
|+++++++++ | 18% ~01s
|++++++++++ | 19% ~01s
|+++++++++++ | 20% ~01s
|+++++++++++ | 21% ~01s
|++++++++++++ | 23% ~01s
|++++++++++++ | 24% ~01s
|+++++++++++++ | 25% ~01s
|++++++++++++++ | 26% ~01s
|++++++++++++++ | 27% ~01s
|+++++++++++++++ | 29% ~01s
|+++++++++++++++ | 30% ~01s
|++++++++++++++++ | 31% ~01s
|+++++++++++++++++ | 32% ~01s
|+++++++++++++++++ | 33% ~01s
|++++++++++++++++++ | 35% ~01s
|++++++++++++++++++ | 36% ~01s
|+++++++++++++++++++ | 37% ~01s
|++++++++++++++++++++ | 38% ~01s
|++++++++++++++++++++ | 39% ~01s
|+++++++++++++++++++++ | 40% ~01s
|+++++++++++++++++++++ | 42% ~01s
|++++++++++++++++++++++ | 43% ~01s
|+++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|++++++++++++++++++++++++ | 46% ~01s
|++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|+++++++++++++++++++++++++ | 50% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|++++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|+++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|++++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~03s
|+++++++++++++++++++++++++++++++++++++++ | 76% ~03s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~03s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~02s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~02s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~02s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~02s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~02s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~02s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~02s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~01s
|+++++++++++++++++++++++++++++++++++++++++++++ | 88% ~01s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~01s
|++++++++++++++++++++++++++++++++++++++++++++++ | 90% ~01s
|++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~01s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~01s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~01s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=09s Calculating cluster 10
| | 0 % ~calculating
|+ | 1 % ~01s
|++ | 2 % ~01s
|++ | 4 % ~01s
|+++ | 5 % ~01s
|++++ | 6 % ~01s
|++++ | 8 % ~01s
|+++++ | 9 % ~01s
|+++++ | 10% ~01s
|++++++ | 11% ~01s
|+++++++ | 12% ~01s
|+++++++ | 14% ~01s
|++++++++ | 15% ~01s
|+++++++++ | 16% ~01s
|+++++++++ | 18% ~01s
|++++++++++ | 19% ~01s
|++++++++++ | 20% ~01s
|+++++++++++ | 21% ~01s
|++++++++++++ | 22% ~01s
|++++++++++++ | 24% ~01s
|+++++++++++++ | 25% ~01s
|++++++++++++++ | 26% ~01s
|++++++++++++++ | 28% ~01s
|+++++++++++++++ | 29% ~01s
|+++++++++++++++ | 30% ~01s
|++++++++++++++++ | 31% ~01s
|+++++++++++++++++ | 32% ~01s
|+++++++++++++++++ | 34% ~01s
|++++++++++++++++++ | 35% ~01s
|+++++++++++++++++++ | 36% ~01s
|+++++++++++++++++++ | 38% ~01s
|++++++++++++++++++++ | 39% ~01s
|++++++++++++++++++++ | 40% ~01s
|+++++++++++++++++++++ | 41% ~01s
|++++++++++++++++++++++ | 42% ~01s
|++++++++++++++++++++++ | 44% ~01s
|+++++++++++++++++++++++ | 45% ~00s
|++++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|+++++++++++++++++++++++++ | 50% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|+++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|++++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|+++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|+++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=01s Calculating cluster 11
| | 0 % ~calculating
|+ | 1 % ~03s
|++ | 2 % ~03s
|++ | 3 % ~03s
|+++ | 4 % ~03s
|+++ | 5 % ~03s
|++++ | 6 % ~03s
|++++ | 7 % ~03s
|+++++ | 9 % ~03s
|+++++ | 10% ~03s
|++++++ | 11% ~03s
|++++++ | 12% ~03s
|+++++++ | 13% ~03s
|+++++++ | 14% ~03s
|++++++++ | 15% ~03s
|++++++++ | 16% ~03s
|+++++++++ | 17% ~03s
|++++++++++ | 18% ~03s
|++++++++++ | 19% ~03s
|+++++++++++ | 20% ~03s
|+++++++++++ | 21% ~03s
|++++++++++++ | 22% ~02s
|++++++++++++ | 23% ~02s
|+++++++++++++ | 24% ~02s
|+++++++++++++ | 26% ~02s
|++++++++++++++ | 27% ~02s
|++++++++++++++ | 28% ~02s
|+++++++++++++++ | 29% ~02s
|+++++++++++++++ | 30% ~02s
|++++++++++++++++ | 31% ~02s
|++++++++++++++++ | 32% ~02s
|+++++++++++++++++ | 33% ~02s
|++++++++++++++++++ | 34% ~02s
|++++++++++++++++++ | 35% ~02s
|+++++++++++++++++++ | 36% ~02s
|+++++++++++++++++++ | 37% ~02s
|++++++++++++++++++++ | 38% ~02s
|++++++++++++++++++++ | 39% ~02s
|+++++++++++++++++++++ | 40% ~02s
|+++++++++++++++++++++ | 41% ~02s
|++++++++++++++++++++++ | 43% ~02s
|++++++++++++++++++++++ | 44% ~02s
|+++++++++++++++++++++++ | 45% ~02s
|+++++++++++++++++++++++ | 46% ~02s
|++++++++++++++++++++++++ | 47% ~02s
|++++++++++++++++++++++++ | 48% ~02s
|+++++++++++++++++++++++++ | 49% ~02s
|+++++++++++++++++++++++++ | 50% ~02s
|++++++++++++++++++++++++++ | 51% ~02s
|+++++++++++++++++++++++++++ | 52% ~01s
|+++++++++++++++++++++++++++ | 53% ~01s
|++++++++++++++++++++++++++++ | 54% ~01s
|++++++++++++++++++++++++++++ | 55% ~01s
|+++++++++++++++++++++++++++++ | 56% ~01s
|+++++++++++++++++++++++++++++ | 57% ~01s
|++++++++++++++++++++++++++++++ | 59% ~01s
|++++++++++++++++++++++++++++++ | 60% ~01s
|+++++++++++++++++++++++++++++++ | 61% ~01s
|+++++++++++++++++++++++++++++++ | 62% ~01s
|++++++++++++++++++++++++++++++++ | 63% ~01s
|++++++++++++++++++++++++++++++++ | 64% ~01s
|+++++++++++++++++++++++++++++++++ | 65% ~01s
|+++++++++++++++++++++++++++++++++ | 66% ~01s
|++++++++++++++++++++++++++++++++++ | 67% ~01s
|+++++++++++++++++++++++++++++++++++ | 68% ~01s
|+++++++++++++++++++++++++++++++++++ | 69% ~01s
|++++++++++++++++++++++++++++++++++++ | 70% ~01s
|++++++++++++++++++++++++++++++++++++ | 71% ~01s
|+++++++++++++++++++++++++++++++++++++ | 72% ~01s
|+++++++++++++++++++++++++++++++++++++ | 73% ~01s
|++++++++++++++++++++++++++++++++++++++ | 74% ~01s
|++++++++++++++++++++++++++++++++++++++ | 76% ~01s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~01s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~01s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~01s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~01s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~01s
|+++++++++++++++++++++++++++++++++++++++++ | 82% ~01s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~01s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=03s Calculating cluster 12
| | 0 % ~calculating
|+ | 1 % ~02s
|++ | 2 % ~03s
|++ | 3 % ~03s
|+++ | 4 % ~02s
|+++ | 5 % ~02s
|++++ | 7 % ~02s
|++++ | 8 % ~02s
|+++++ | 9 % ~02s
|+++++ | 10% ~02s
|++++++ | 11% ~02s
|+++++++ | 12% ~02s
|+++++++ | 13% ~02s
|++++++++ | 14% ~02s
|++++++++ | 15% ~02s
|+++++++++ | 16% ~02s
|+++++++++ | 18% ~02s
|++++++++++ | 19% ~02s
|++++++++++ | 20% ~02s
|+++++++++++ | 21% ~02s
|+++++++++++ | 22% ~01s
|++++++++++++ | 23% ~01s
|+++++++++++++ | 24% ~01s
|+++++++++++++ | 25% ~01s
|++++++++++++++ | 26% ~01s
|++++++++++++++ | 27% ~01s
|+++++++++++++++ | 29% ~01s
|+++++++++++++++ | 30% ~01s
|++++++++++++++++ | 31% ~01s
|++++++++++++++++ | 32% ~01s
|+++++++++++++++++ | 33% ~01s
|++++++++++++++++++ | 34% ~01s
|++++++++++++++++++ | 35% ~01s
|+++++++++++++++++++ | 36% ~01s
|+++++++++++++++++++ | 37% ~01s
|++++++++++++++++++++ | 38% ~01s
|++++++++++++++++++++ | 40% ~01s
|+++++++++++++++++++++ | 41% ~01s
|+++++++++++++++++++++ | 42% ~01s
|++++++++++++++++++++++ | 43% ~01s
|++++++++++++++++++++++ | 44% ~01s
|+++++++++++++++++++++++ | 45% ~01s
|++++++++++++++++++++++++ | 46% ~01s
|++++++++++++++++++++++++ | 47% ~01s
|+++++++++++++++++++++++++ | 48% ~01s
|+++++++++++++++++++++++++ | 49% ~01s
|++++++++++++++++++++++++++ | 51% ~01s
|++++++++++++++++++++++++++ | 52% ~01s
|+++++++++++++++++++++++++++ | 53% ~01s
|+++++++++++++++++++++++++++ | 54% ~01s
|++++++++++++++++++++++++++++ | 55% ~01s
|+++++++++++++++++++++++++++++ | 56% ~01s
|+++++++++++++++++++++++++++++ | 57% ~01s
|++++++++++++++++++++++++++++++ | 58% ~01s
|++++++++++++++++++++++++++++++ | 59% ~01s
|+++++++++++++++++++++++++++++++ | 60% ~01s
|+++++++++++++++++++++++++++++++ | 62% ~01s
|++++++++++++++++++++++++++++++++ | 63% ~01s
|++++++++++++++++++++++++++++++++ | 64% ~01s
|+++++++++++++++++++++++++++++++++ | 65% ~01s
|+++++++++++++++++++++++++++++++++ | 66% ~01s
|++++++++++++++++++++++++++++++++++ | 67% ~01s
|+++++++++++++++++++++++++++++++++++ | 68% ~01s
|+++++++++++++++++++++++++++++++++++ | 69% ~01s
|++++++++++++++++++++++++++++++++++++ | 70% ~01s
|++++++++++++++++++++++++++++++++++++ | 71% ~01s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=02s Calculating cluster 13
| | 0 % ~calculating
|+ | 1 % ~01s
|+ | 2 % ~01s
|++ | 3 % ~01s
|++ | 4 % ~01s
|+++ | 5 % ~01s
|+++ | 6 % ~01s
|++++ | 7 % ~01s
|++++ | 8 % ~01s
|+++++ | 9 % ~01s
|+++++ | 10% ~01s
|++++++ | 11% ~01s
|++++++ | 12% ~01s
|+++++++ | 13% ~01s
|+++++++ | 14% ~01s
|++++++++ | 15% ~01s
|++++++++ | 16% ~01s
|+++++++++ | 17% ~01s
|+++++++++ | 18% ~01s
|++++++++++ | 19% ~01s
|++++++++++ | 20% ~01s
|+++++++++++ | 21% ~01s
|+++++++++++ | 22% ~01s
|++++++++++++ | 23% ~01s
|++++++++++++ | 24% ~01s
|+++++++++++++ | 25% ~01s
|+++++++++++++ | 26% ~01s
|++++++++++++++ | 27% ~01s
|++++++++++++++ | 28% ~01s
|+++++++++++++++ | 29% ~01s
|+++++++++++++++ | 30% ~01s
|++++++++++++++++ | 31% ~01s
|++++++++++++++++ | 32% ~01s
|+++++++++++++++++ | 33% ~01s
|+++++++++++++++++ | 34% ~01s
|++++++++++++++++++ | 35% ~01s
|++++++++++++++++++ | 36% ~01s
|+++++++++++++++++++ | 37% ~01s
|+++++++++++++++++++ | 38% ~01s
|++++++++++++++++++++ | 39% ~01s
|++++++++++++++++++++ | 40% ~01s
|+++++++++++++++++++++ | 41% ~01s
|+++++++++++++++++++++ | 42% ~01s
|++++++++++++++++++++++ | 43% ~01s
|++++++++++++++++++++++ | 44% ~01s
|+++++++++++++++++++++++ | 45% ~01s
|+++++++++++++++++++++++ | 46% ~01s
|++++++++++++++++++++++++ | 47% ~01s
|++++++++++++++++++++++++ | 48% ~01s
|+++++++++++++++++++++++++ | 49% ~01s
|+++++++++++++++++++++++++ | 50% ~01s
|++++++++++++++++++++++++++ | 51% ~01s
|++++++++++++++++++++++++++ | 52% ~01s
|+++++++++++++++++++++++++++ | 53% ~01s
|+++++++++++++++++++++++++++ | 54% ~01s
|++++++++++++++++++++++++++++ | 55% ~01s
|++++++++++++++++++++++++++++ | 56% ~01s
|+++++++++++++++++++++++++++++ | 57% ~01s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|+++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|+++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=01s Loco.markers %>%
group_by(cluster) %>%
slice_max(n = 10, order_by = avg_log2FC)Loco.markers %>%
group_by(cluster) %>%
top_n(n = 10, wt = avg_log2FC) -> top10
DoHeatmap(Loco, features = top10$gene) + NoLegend()
Locon <- subset(Loco, idents = c(1,4,5,8,10,11,13))
RunPCA(Locon, npcs = 30, verbose = FALSE) %>%
RunUMAP(dims = 1:30, verbose = FALSE) %>%
FindNeighbors(reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)An object of class Seurat
34320 features across 1127 samples within 2 assays
Active assay: SCT (16437 features, 10000 variable features)
1 other assay present: RNA
2 dimensional reductions calculated: pca, umapDimHeatmap(Locon, dims = 1:20, cells = 500, balanced = TRUE)
Loco.markers %>%
group_by(cluster) %>%
top_n(n = 10, wt = avg_log2FC) -> top10
DoHeatmap(Locon, features = top10$gene) + NoLegend()
Locon2 <- SCTransform(Locon, return.only.var.genes = FALSE, verbose = FALSE) %>%
RunPCA(npcs = 30, verbose = FALSE) %>%
RunUMAP( dims = 1:30, verbose = FALSE) %>%
FindNeighbors(reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)
DimHeatmap(Locon2, dims = 1:20, cells = 500, balanced = TRUE)
DimPlot(Locon2, reduction = "umap")
FeaturePlot(Locon2, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Hcrtr1", "Eya2", "Th", "Dbh"), order = T)
LoconTH <- subset(Loco, idents = 8)
LoconTH <- FindNeighbors(LoconTH, reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)%>%
RunUMAP( dims = 1:30, verbose = FALSE)
DimHeatmap(LoconTH, dims = 1:20, cells = 500, balanced = TRUE)Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
ElbowPlot(LoconTH, ndims = 30)
DimPlot(LoconTH, reduction = "umap")
FeaturePlot(LoconTH, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Th"), order = T)
Locon3 <- subset(Locon2, idents = 5)
RunPCA(Locon2, npcs = 30, verbose = FALSE) %>%
RunUMAP(dims = 1:30, verbose = FALSE) %>%
FindNeighbors(reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)An object of class Seurat
32614 features across 1151 samples within 2 assays
Active assay: SCT (14731 features, 3000 variable features)
1 other assay present: RNA
2 dimensional reductions calculated: pca, umapDimHeatmap(Locon3, dims = 1:20, cells = 500, balanced = TRUE)Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
Warning: Requested number is larger than the number of available items (82). Setting to 82.
FeaturePlot(Locon3, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Th", "Slc18a2", "Dbh"), order = T)
Locon3th <- subset(Locon3, Slc18a2 > 1)FeaturePlot(Locon3th, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Th", "Slc18a2"), order = T)
Locon4 <- FindNeighbors(Locon, reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE) %>%
RunUMAP( dims = 1:30, verbose = FALSE)
DimHeatmap(Locon4, dims = 1:20, cells = 500, balanced = TRUE)
DimPlot(Locon4, reduction = "umap")
FeaturePlot(Locon4, features = c("Gal", "Grp", "Sst", "Slc17a6", "Pdyn", "Cartpt", "Bdnf", "Th", "Slc18a2", "Dbh", "Gad1", "Calca"), order = T)
SeuratDisk::SaveH5Seurat(Locon4,
filename = paste0("./data/",
Project(object = Locon4),
".h5Seurat"),
overwrite = TRUE)Registered S3 method overwritten by 'SeuratDisk':
method from
as.sparse.H5Group Seurat
Warning: Overwriting previous file ./data/Locus_Coruleus.h5Seurat
Creating h5Seurat file for version 3.1.5.9900
Adding counts for RNA
Adding data for RNA
No variable features found for RNA
No feature-level metadata found for RNA
Adding counts for SCT
Adding data for SCT
Adding scale.data for SCT
Adding variable features for SCT
No feature-level metadata found for SCT
Writing out SCTModel.list for SCT
Adding cell embeddings for pca
Adding loadings for pca
No projected loadings for pca
Adding standard deviations for pca
No JackStraw data for pca
Adding cell embeddings for umap
No loadings for umap
No projected loadings for umap
No standard deviations for umap
No JackStraw data for umapFeaturePlot(Locon4, slot = "scale.data", features = c("Slc17a6", "Slc17a7", "Gad1", "Gad2", "Slc32a1", "Dbh", "Th", "Slc6a2", "Slc18a2"), order = T)
FeaturePlot(Locon4, slot = "scale.data", features = c("Gal", "Grp", "Calca", "Cartpt", "Pdyn", "Bdnf", "Adcyap1", "Sst", "Npy"), order = T)
FeaturePlot(Locon4, slot = "scale.data", features = c("Hcrtr1", "Sstr2", "Calcr", "Galr1"), order = T)
LoconTH4 <- subset(Locon4, idents = 3)
LoconTH4 <- FindNeighbors(LoconTH4, reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)%>%
RunUMAP( dims = 1:30, verbose = FALSE)
DimHeatmap(LoconTH4, dims = 1:20, cells = 500, balanced = TRUE)Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
Warning: Requested number is larger than the number of available items (80). Setting to 80.
FeaturePlot(Locon4, slot = "scale.data", features = c("Phox2a", "Phox2b", "Eya1", "Eya2"), order = T)
DimPlot(LoconTH4, reduction = "umap")
DimPlot(Locon4, reduction = “umap”)
FeaturePlot(LoconTH4, slot = "scale.data", features = c("Phox2a", "Phox2b", "Eya1", "Eya2"), order = T)
FeaturePlot(LoconTH4, slot = "scale.data", features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Calca", "Hcrtr1", "Sstr2", "Calcr", "Slc6a2", "Slc18a2", "Dbh"), order = T)
FeaturePlot(LoconTH4, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Calca", "Npy", "Hcrtr1", "Sstr2", "Calcr", "Galr1", "Dbh"), order = T)
Warning in FeaturePlot(LoconTH4, features = c("Gal", "Grp", "Pdyn", "Cartpt", :
All cells have the same value (0) of Galr1.
Locon5 <- FindNeighbors(Locon, reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
FindClusters(resolution = 2, algorithm = 1, verbose = FALSE) %>%
RunUMAP( dims = 1:30, verbose = FALSE)
DimHeatmap(Locon5, dims = 1:20, cells = 500, balanced = TRUE)
Locon5.markers <- FindAllMarkers(Locon5, only.pos = TRUE, test.use = "wilcox", min.pct = 0.1, logfc.threshold = 0.25)Calculating cluster 0
| | 0 % ~calculating
|+++ | 5 % ~00s
|++++++ | 11% ~00s
|++++++++ | 16% ~00s
|+++++++++++ | 21% ~00s
|++++++++++++++ | 26% ~00s
|++++++++++++++++ | 32% ~00s
|+++++++++++++++++++ | 37% ~00s
|++++++++++++++++++++++ | 42% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 1
| | 0 % ~calculating
|+++ | 4 % ~00s
|+++++ | 9 % ~00s
|+++++++ | 13% ~00s
|+++++++++ | 17% ~00s
|+++++++++++ | 22% ~00s
|++++++++++++++ | 26% ~00s
|++++++++++++++++ | 30% ~00s
|++++++++++++++++++ | 35% ~00s
|++++++++++++++++++++ | 39% ~00s
|++++++++++++++++++++++ | 43% ~00s
|++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|+++++++++++++++++++++++++++++++++++ | 70% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 2
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 2 % ~00s
|++ | 3 % ~00s
|+++ | 5 % ~00s
|+++ | 6 % ~00s
|++++ | 7 % ~00s
|++++ | 8 % ~00s
|+++++ | 9 % ~00s
|++++++ | 10% ~00s
|++++++ | 11% ~00s
|+++++++ | 12% ~00s
|+++++++ | 14% ~00s
|++++++++ | 15% ~00s
|++++++++ | 16% ~00s
|+++++++++ | 17% ~00s
|++++++++++ | 18% ~00s
|++++++++++ | 19% ~00s
|+++++++++++ | 20% ~00s
|+++++++++++ | 22% ~00s
|++++++++++++ | 23% ~00s
|++++++++++++ | 24% ~00s
|+++++++++++++ | 25% ~00s
|++++++++++++++ | 26% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 28% ~00s
|+++++++++++++++ | 30% ~00s
|++++++++++++++++ | 31% ~00s
|++++++++++++++++ | 32% ~00s
|+++++++++++++++++ | 33% ~00s
|++++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 35% ~00s
|+++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 38% ~00s
|++++++++++++++++++++ | 39% ~00s
|++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|++++++++++++++++++++++ | 42% ~00s
|++++++++++++++++++++++ | 43% ~00s
|+++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|+++++++++++++++++++++++++ | 50% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|+++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|+++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|++++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 3
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 2 % ~00s
|++ | 4 % ~00s
|+++ | 5 % ~00s
|+++ | 6 % ~00s
|++++ | 7 % ~00s
|+++++ | 8 % ~00s
|+++++ | 9 % ~00s
|++++++ | 11% ~00s
|++++++ | 12% ~00s
|+++++++ | 13% ~00s
|++++++++ | 14% ~00s
|++++++++ | 15% ~00s
|+++++++++ | 16% ~00s
|+++++++++ | 18% ~00s
|++++++++++ | 19% ~00s
|++++++++++ | 20% ~00s
|+++++++++++ | 21% ~00s
|++++++++++++ | 22% ~00s
|++++++++++++ | 24% ~00s
|+++++++++++++ | 25% ~00s
|+++++++++++++ | 26% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 28% ~00s
|+++++++++++++++ | 29% ~00s
|++++++++++++++++ | 31% ~00s
|++++++++++++++++ | 32% ~00s
|+++++++++++++++++ | 33% ~00s
|++++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 35% ~00s
|+++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 38% ~00s
|++++++++++++++++++++ | 39% ~00s
|++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|++++++++++++++++++++++ | 42% ~00s
|++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|+++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|+++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|++++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|+++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|+++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|++++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 4
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 3 % ~00s
|+++ | 4 % ~00s
|+++ | 6 % ~00s
|++++ | 7 % ~00s
|+++++ | 9 % ~00s
|++++++ | 10% ~00s
|++++++ | 12% ~00s
|+++++++ | 13% ~00s
|++++++++ | 15% ~00s
|+++++++++ | 16% ~00s
|+++++++++ | 18% ~00s
|++++++++++ | 19% ~00s
|+++++++++++ | 21% ~00s
|++++++++++++ | 22% ~00s
|++++++++++++ | 24% ~00s
|+++++++++++++ | 25% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 28% ~00s
|+++++++++++++++ | 30% ~00s
|++++++++++++++++ | 31% ~00s
|+++++++++++++++++ | 33% ~00s
|++++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 37% ~00s
|++++++++++++++++++++ | 39% ~00s
|+++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 42% ~00s
|++++++++++++++++++++++ | 43% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|++++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|++++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|++++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 5
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 2 % ~00s
|++ | 4 % ~00s
|+++ | 5 % ~00s
|++++ | 6 % ~00s
|++++ | 7 % ~00s
|+++++ | 9 % ~00s
|+++++ | 10% ~00s
|++++++ | 11% ~00s
|+++++++ | 12% ~00s
|+++++++ | 13% ~00s
|++++++++ | 15% ~00s
|++++++++ | 16% ~00s
|+++++++++ | 17% ~00s
|++++++++++ | 18% ~00s
|++++++++++ | 20% ~00s
|+++++++++++ | 21% ~00s
|+++++++++++ | 22% ~00s
|++++++++++++ | 23% ~00s
|+++++++++++++ | 24% ~00s
|+++++++++++++ | 26% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 28% ~00s
|+++++++++++++++ | 29% ~00s
|++++++++++++++++ | 30% ~00s
|++++++++++++++++ | 32% ~00s
|+++++++++++++++++ | 33% ~00s
|++++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 35% ~00s
|+++++++++++++++++++ | 37% ~00s
|+++++++++++++++++++ | 38% ~00s
|++++++++++++++++++++ | 39% ~00s
|+++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|++++++++++++++++++++++ | 43% ~00s
|++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|++++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|+++++++++++++++++++++++++ | 50% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|+++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|+++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|++++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 6
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 2 % ~00s
|++ | 3 % ~00s
|+++ | 4 % ~00s
|+++ | 5 % ~00s
|++++ | 6 % ~00s
|++++ | 7 % ~00s
|+++++ | 8 % ~00s
|+++++ | 9 % ~00s
|++++++ | 11% ~00s
|++++++ | 12% ~00s
|+++++++ | 13% ~00s
|+++++++ | 14% ~00s
|++++++++ | 15% ~00s
|++++++++ | 16% ~00s
|+++++++++ | 17% ~00s
|+++++++++ | 18% ~00s
|++++++++++ | 19% ~00s
|++++++++++ | 20% ~00s
|+++++++++++ | 21% ~00s
|++++++++++++ | 22% ~00s
|++++++++++++ | 23% ~00s
|+++++++++++++ | 24% ~00s
|+++++++++++++ | 25% ~00s
|++++++++++++++ | 26% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 28% ~00s
|+++++++++++++++ | 29% ~00s
|++++++++++++++++ | 31% ~00s
|++++++++++++++++ | 32% ~00s
|+++++++++++++++++ | 33% ~00s
|+++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 35% ~00s
|++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 37% ~00s
|+++++++++++++++++++ | 38% ~00s
|++++++++++++++++++++ | 39% ~00s
|++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|++++++++++++++++++++++ | 42% ~00s
|++++++++++++++++++++++ | 43% ~00s
|+++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|++++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|+++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|+++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|++++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|+++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 7
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 3 % ~00s
|++ | 4 % ~00s
|+++ | 5 % ~00s
|++++ | 6 % ~00s
|++++ | 8 % ~00s
|+++++ | 9 % ~00s
|++++++ | 10% ~00s
|++++++ | 12% ~00s
|+++++++ | 13% ~00s
|++++++++ | 14% ~00s
|++++++++ | 15% ~00s
|+++++++++ | 17% ~00s
|+++++++++ | 18% ~00s
|++++++++++ | 19% ~00s
|+++++++++++ | 21% ~00s
|+++++++++++ | 22% ~00s
|++++++++++++ | 23% ~00s
|+++++++++++++ | 24% ~00s
|+++++++++++++ | 26% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 28% ~00s
|+++++++++++++++ | 29% ~00s
|++++++++++++++++ | 31% ~00s
|+++++++++++++++++ | 32% ~00s
|+++++++++++++++++ | 33% ~00s
|++++++++++++++++++ | 35% ~00s
|++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 37% ~00s
|++++++++++++++++++++ | 38% ~00s
|++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|++++++++++++++++++++++ | 42% ~00s
|++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|++++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|+++++++++++++++++++++++++ | 50% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|+++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|+++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|+++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|++++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 8
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 3 % ~00s
|+++ | 4 % ~00s
|+++ | 6 % ~00s
|++++ | 7 % ~00s
|+++++ | 9 % ~00s
|+++++ | 10% ~00s
|++++++ | 11% ~00s
|+++++++ | 13% ~00s
|++++++++ | 14% ~00s
|++++++++ | 16% ~00s
|+++++++++ | 17% ~00s
|++++++++++ | 19% ~00s
|++++++++++ | 20% ~00s
|+++++++++++ | 21% ~00s
|++++++++++++ | 23% ~00s
|+++++++++++++ | 24% ~00s
|+++++++++++++ | 26% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 29% ~00s
|+++++++++++++++ | 30% ~00s
|++++++++++++++++ | 31% ~00s
|+++++++++++++++++ | 33% ~00s
|++++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 37% ~00s
|++++++++++++++++++++ | 39% ~00s
|++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|++++++++++++++++++++++ | 43% ~00s
|+++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|+++++++++++++++++++++++++ | 50% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|++++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|+++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|++++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 9
| | 0 % ~calculating
|+ | 1 % ~01s
|++ | 2 % ~01s
|++ | 3 % ~01s
|+++ | 4 % ~01s
|+++ | 5 % ~01s
|++++ | 6 % ~01s
|++++ | 7 % ~01s
|+++++ | 8 % ~01s
|+++++ | 9 % ~01s
|++++++ | 10% ~01s
|++++++ | 11% ~01s
|+++++++ | 12% ~01s
|+++++++ | 13% ~01s
|++++++++ | 14% ~01s
|++++++++ | 15% ~01s
|+++++++++ | 16% ~01s
|+++++++++ | 17% ~01s
|++++++++++ | 18% ~01s
|++++++++++ | 19% ~01s
|+++++++++++ | 20% ~01s
|+++++++++++ | 21% ~01s
|++++++++++++ | 22% ~01s
|++++++++++++ | 23% ~01s
|+++++++++++++ | 24% ~01s
|+++++++++++++ | 25% ~01s
|++++++++++++++ | 26% ~01s
|++++++++++++++ | 27% ~01s
|+++++++++++++++ | 28% ~01s
|+++++++++++++++ | 29% ~01s
|++++++++++++++++ | 30% ~01s
|++++++++++++++++ | 31% ~01s
|+++++++++++++++++ | 32% ~01s
|+++++++++++++++++ | 33% ~01s
|++++++++++++++++++ | 34% ~01s
|++++++++++++++++++ | 35% ~01s
|+++++++++++++++++++ | 36% ~01s
|+++++++++++++++++++ | 37% ~01s
|++++++++++++++++++++ | 38% ~01s
|++++++++++++++++++++ | 39% ~01s
|+++++++++++++++++++++ | 40% ~01s
|+++++++++++++++++++++ | 41% ~01s
|++++++++++++++++++++++ | 42% ~01s
|++++++++++++++++++++++ | 43% ~01s
|+++++++++++++++++++++++ | 44% ~01s
|+++++++++++++++++++++++ | 45% ~01s
|++++++++++++++++++++++++ | 46% ~01s
|++++++++++++++++++++++++ | 47% ~01s
|+++++++++++++++++++++++++ | 48% ~01s
|+++++++++++++++++++++++++ | 49% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|+++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|+++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|+++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 88% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 92% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 95% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 98% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=01s Calculating cluster 10
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 3 % ~00s
|+++ | 4 % ~00s
|+++ | 6 % ~00s
|++++ | 7 % ~00s
|+++++ | 9 % ~00s
|+++++ | 10% ~00s
|++++++ | 11% ~00s
|+++++++ | 13% ~00s
|++++++++ | 14% ~00s
|++++++++ | 16% ~00s
|+++++++++ | 17% ~00s
|++++++++++ | 19% ~00s
|++++++++++ | 20% ~00s
|+++++++++++ | 21% ~00s
|++++++++++++ | 23% ~00s
|+++++++++++++ | 24% ~00s
|+++++++++++++ | 26% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 29% ~00s
|+++++++++++++++ | 30% ~00s
|++++++++++++++++ | 31% ~00s
|+++++++++++++++++ | 33% ~00s
|++++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 37% ~00s
|++++++++++++++++++++ | 39% ~00s
|++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|++++++++++++++++++++++ | 43% ~00s
|+++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|+++++++++++++++++++++++++ | 50% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|++++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|+++++++++++++++++++++++++++++++++++ | 70% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|++++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 86% ~00s
|++++++++++++++++++++++++++++++++++++++++++++ | 87% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 89% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++ | 90% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++ | 91% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++ | 93% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 94% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 11
| | 0 % ~calculating
|+ | 1 % ~00s
|++ | 2 % ~00s
|++ | 3 % ~00s
|+++ | 4 % ~00s
|+++ | 5 % ~00s
|++++ | 6 % ~00s
|++++ | 7 % ~00s
|+++++ | 8 % ~00s
|+++++ | 9 % ~00s
|++++++ | 11% ~00s
|++++++ | 12% ~00s
|+++++++ | 13% ~00s
|+++++++ | 14% ~00s
|++++++++ | 15% ~00s
|++++++++ | 16% ~00s
|+++++++++ | 17% ~00s
|+++++++++ | 18% ~00s
|++++++++++ | 19% ~00s
|++++++++++ | 20% ~00s
|+++++++++++ | 21% ~00s
|++++++++++++ | 22% ~00s
|++++++++++++ | 23% ~00s
|+++++++++++++ | 24% ~00s
|+++++++++++++ | 25% ~00s
|++++++++++++++ | 26% ~00s
|++++++++++++++ | 27% ~00s
|+++++++++++++++ | 28% ~00s
|+++++++++++++++ | 29% ~00s
|++++++++++++++++ | 31% ~00s
|++++++++++++++++ | 32% ~00s
|+++++++++++++++++ | 33% ~00s
|+++++++++++++++++ | 34% ~00s
|++++++++++++++++++ | 35% ~00s
|++++++++++++++++++ | 36% ~00s
|+++++++++++++++++++ | 37% ~00s
|+++++++++++++++++++ | 38% ~00s
|++++++++++++++++++++ | 39% ~00s
|++++++++++++++++++++ | 40% ~00s
|+++++++++++++++++++++ | 41% ~00s
|++++++++++++++++++++++ | 42% ~00s
|++++++++++++++++++++++ | 43% ~00s
|+++++++++++++++++++++++ | 44% ~00s
|+++++++++++++++++++++++ | 45% ~00s
|++++++++++++++++++++++++ | 46% ~00s
|++++++++++++++++++++++++ | 47% ~00s
|+++++++++++++++++++++++++ | 48% ~00s
|+++++++++++++++++++++++++ | 49% ~00s
|++++++++++++++++++++++++++ | 51% ~00s
|++++++++++++++++++++++++++ | 52% ~00s
|+++++++++++++++++++++++++++ | 53% ~00s
|+++++++++++++++++++++++++++ | 54% ~00s
|++++++++++++++++++++++++++++ | 55% ~00s
|++++++++++++++++++++++++++++ | 56% ~00s
|+++++++++++++++++++++++++++++ | 57% ~00s
|+++++++++++++++++++++++++++++ | 58% ~00s
|++++++++++++++++++++++++++++++ | 59% ~00s
|++++++++++++++++++++++++++++++ | 60% ~00s
|+++++++++++++++++++++++++++++++ | 61% ~00s
|++++++++++++++++++++++++++++++++ | 62% ~00s
|++++++++++++++++++++++++++++++++ | 63% ~00s
|+++++++++++++++++++++++++++++++++ | 64% ~00s
|+++++++++++++++++++++++++++++++++ | 65% ~00s
|++++++++++++++++++++++++++++++++++ | 66% ~00s
|++++++++++++++++++++++++++++++++++ | 67% ~00s
|+++++++++++++++++++++++++++++++++++ | 68% ~00s
|+++++++++++++++++++++++++++++++++++ | 69% ~00s
|++++++++++++++++++++++++++++++++++++ | 71% ~00s
|++++++++++++++++++++++++++++++++++++ | 72% ~00s
|+++++++++++++++++++++++++++++++++++++ | 73% ~00s
|+++++++++++++++++++++++++++++++++++++ | 74% ~00s
|++++++++++++++++++++++++++++++++++++++ | 75% ~00s
|++++++++++++++++++++++++++++++++++++++ | 76% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 77% ~00s
|+++++++++++++++++++++++++++++++++++++++ | 78% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 79% ~00s
|++++++++++++++++++++++++++++++++++++++++ | 80% ~00s
|+++++++++++++++++++++++++++++++++++++++++ | 81% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 82% ~00s
|++++++++++++++++++++++++++++++++++++++++++ | 83% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 84% ~00s
|+++++++++++++++++++++++++++++++++++++++++++ | 85% ~00s
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|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 12
| | 0 % ~calculating
|+ | 1 % ~00s
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|++++++++++++++++++++++++++++++++++++++++++++++++ | 96% ~00s
|+++++++++++++++++++++++++++++++++++++++++++++++++ | 97% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 99% ~00s
|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 13
| | 0 % ~calculating
|+ | 1 % ~01s
|++ | 2 % ~01s
|++ | 3 % ~01s
|+++ | 4 % ~01s
|+++ | 6 % ~02s
|++++ | 7 % ~02s
|++++ | 8 % ~01s
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|++++++++ | 15% ~01s
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|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=01s Calculating cluster 14
| | 0 % ~calculating
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|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 15
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|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Calculating cluster 16
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|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=01s Calculating cluster 17
| | 0 % ~calculating
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|++++++++++++++++++++++++++++++++++++++++++++++++++| 100% elapsed=00s Locon5.markers %>%
group_by(cluster) %>%
slice_max(n = 10, order_by = avg_log2FC)
LoconTH4.markers <- FindAllMarkers(LoconTH4, only.pos = TRUE, test.use = "MAST", min.pct = 0.1, logfc.threshold = 0.25)
LoconTH4.markers %>%
group_by(cluster) %>%
slice_max(n = 10, order_by = avg_log2FC)LoconTH4.markers <- FindAllMarkers(LoconTH4, only.pos = TRUE, test.use = "LR")
LoconTH4.markers %>%
group_by(cluster) %>%
slice_max(n = 10, order_by = avg_log2FC)
LoconTH4.markers %>% filter(gene == "Grp")LoconTH4.markers <- FindAllMarkers(LoconTH4, only.pos = TRUE, test.use = "roc", min.pct = 0.1)
LoconTH4.markers %>%
group_by(cluster) %>%
slice_max(n = 10, order_by = avg_log2FC)Add a new chunk by clicking the Insert Chunk button on the toolbar or by pressing Ctrl+Alt+I.When you save the notebook, an HTML file containing the code and output will be saved alongside it (click the Preview button or press Ctrl+Shift+K to preview the HTML file).
The preview shows you a rendered HTML copy of the contents of the editor. Consequently, unlike Knit, Preview does not run any R code chunks. Instead, the output of the chunk when it was last run in the editor is displayed.
---
title: "R Notebook"
output: html_notebook
---

This is an [R Markdown](http://rmarkdown.rstudio.com) Notebook. When you execute code within the notebook, the results appear beneath the code. 

Try executing this chunk by clicking the *Run* button within the chunk or by placing your cursor inside it and pressing *Ctrl+Shift+Enter*. 

```{r Data loading QC Violin}
rm(list = ls())
library(Seurat)
library(dplyr)
library(patchwork)
library(magrittr)
# Load the LC dataset
Loco.data <- Read10X(data.dir = "~/data/Locus_Coruleus2/count/")
# Initialize the Seurat object with the raw (non-normalized data).
Loco <- CreateSeuratObject(counts = Loco.data, project = "Locus_Coruleus", min.cells = 3, min.features = 200)
# The [[ operator can add columns to object metadata. This is a great place to stash QC stats
Loco[["percent.mt"]] <- PercentageFeatureSet(Loco, pattern = "^mt-")
# Visualize QC metrics as a violin plot
VlnPlot(Loco, features = c("nFeature_RNA", "nCount_RNA", "percent.mt"), ncol = 3)
# FeatureScatter is typically used to visualize feature-feature relationships, but can be used
# for anything calculated by the object, i.e. columns in object metadata, PC scores etc.

```

```{r QC2 scatter, fig.width=8, fig.asp=0.618}
plot1 <- FeatureScatter(Loco, feature1 = "nCount_RNA", feature2 = "percent.mt")
plot2 <- FeatureScatter(Loco, feature1 = "nCount_RNA", feature2 = "nFeature_RNA")
plot1 + plot2
```

```{r SCTransform pipeline, fig.asp=0.618, fig.width=12}
Loco <- subset(Loco, subset = nFeature_RNA > 200 & percent.mt < 20)
# normalize and run dimensionality reduction on control dataset
Loco <- SCTransform(Loco, return.only.var.genes = FALSE, verbose = FALSE, variable.features.n = 10000) %>%
    RunPCA(npcs = 100, verbose = FALSE) %>%
    RunUMAP(dims = 1:20, verbose = FALSE) %>%
    FindNeighbors(reduction = "pca", dims = 1:100, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
    FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)
DimHeatmap(Loco, dims = 1:20, cells = 500, balanced = TRUE)
```


```{r elbow PCA}
ElbowPlot(Loco, ndims = 30)
```


```{r Umap all clusters resolution 0.3}
DimPlot(Loco, reduction = "umap",label = T)
```

```{r glutamate marker}
FeaturePlot(Loco, features = c("Slc17a7", "Slc18a2", "Slc17a6", "Gad2", "Piezo2"), order = T)
```


```{r Markers}
Loco.markers <- FindAllMarkers(Loco, only.pos = TRUE, test.use = "wilcox", min.pct = 0.1, logfc.threshold = 0.25)
Loco.markers %>%
    group_by(cluster) %>%
    slice_max(n = 10, order_by = avg_log2FC)
```
```{r}
Loco.markers %>%
    group_by(cluster) %>%
    top_n(n = 10, wt = avg_log2FC) -> top10
DoHeatmap(Loco, features = top10$gene) + NoLegend()
```

```{r neuronal subset}
Locon <- subset(Loco, idents = c(1,4,5,8,10,11,13))
    RunPCA(Locon, npcs = 30, verbose = FALSE) %>%
    RunUMAP(dims = 1:30, verbose = FALSE) %>%
    FindNeighbors(reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
    FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)
DimHeatmap(Locon, dims = 1:20, cells = 500, balanced = TRUE)
```

```{r UMAP neurons}
DimPlot(Locon, reduction = "umap")
```
```{r}
Loco.markers %>%
    group_by(cluster) %>%
    top_n(n = 10, wt = avg_log2FC) -> top10
DoHeatmap(Locon, features = top10$gene) + NoLegend()
```

```{r}

Locon2 <- SCTransform(Locon, return.only.var.genes = FALSE, verbose = FALSE) %>%
    RunPCA(npcs = 30, verbose = FALSE) %>%
    RunUMAP( dims = 1:30, verbose = FALSE) %>%
    FindNeighbors(reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
    FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)
DimHeatmap(Locon2, dims = 1:20, cells = 500, balanced = TRUE)
```

```{r}
DimPlot(Locon2, reduction = "umap")
```
```{r}
FeaturePlot(Locon2, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Hcrtr1", "Eya2", "Th", "Dbh"), order = T)
```
```{r}
LoconTH <- subset(Loco, idents = 8)
LoconTH <- FindNeighbors(LoconTH, reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
    FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)%>%
    RunUMAP( dims = 1:30, verbose = FALSE) 
DimHeatmap(LoconTH, dims = 1:20, cells = 500, balanced = TRUE)
```
```{r}
ElbowPlot(LoconTH, ndims = 30)
```
```{r}
DimPlot(LoconTH, reduction = "umap")
```
```{r}
FeaturePlot(LoconTH, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Th"), order = T)
```

```{r}
Locon3 <- subset(Locon2, idents = 5)
    RunPCA(Locon2, npcs = 30, verbose = FALSE) %>%
    RunUMAP(dims = 1:30, verbose = FALSE) %>%
    FindNeighbors(reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
    FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)
DimHeatmap(Locon3, dims = 1:20, cells = 500, balanced = TRUE)
```
```{r}
FeaturePlot(Locon3, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Th", "Slc18a2", "Dbh"), order = T)
```
```{r}
Locon3th <- subset(Locon3, Slc18a2 > 1)
```

```{r}
FeaturePlot(Locon3th, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Th", "Slc18a2"), order = T)
```
```{r}
Locon4 <- FindNeighbors(Locon, reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
    FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE) %>%
    RunUMAP( dims = 1:30, verbose = FALSE)
DimHeatmap(Locon4, dims = 1:20, cells = 500, balanced = TRUE)
```
```{r}
DimPlot(Locon4, reduction = "umap")
```
```{r}
FeaturePlot(Locon4, features = c("Gal", "Grp", "Sst", "Slc17a6", "Pdyn", "Cartpt", "Bdnf", "Th", "Slc18a2", "Dbh", "Gad1", "Calca"), order = T)
SeuratDisk::SaveH5Seurat(Locon4, 
                         filename = paste0("./data/",
                                           Project(object = Locon4), 
                                           ".h5Seurat"), 
                         overwrite = TRUE)
```
```{r}
FeaturePlot(Locon4, slot = "scale.data", features = c("Slc17a6", "Slc17a7", "Gad1", "Gad2", "Slc32a1", "Dbh", "Th", "Slc6a2", "Slc18a2"), order = T)
```


```{r}
FeaturePlot(Locon4, slot = "scale.data", features = c("Gal", "Grp", "Calca", "Cartpt", "Pdyn", "Bdnf", "Adcyap1", "Sst", "Npy"), order = T)
```



```{r}
FeaturePlot(Locon4, slot = "scale.data", features = c("Hcrtr1", "Sstr2", "Calcr", "Galr1"), order = T)
```


```{r}
LoconTH4 <- subset(Locon4, idents = 3)
LoconTH4 <- FindNeighbors(LoconTH4, reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
    FindClusters(resolution = 0.3, algorithm = 1, verbose = FALSE)%>%
    RunUMAP( dims = 1:30, verbose = FALSE) 
DimHeatmap(LoconTH4, dims = 1:20, cells = 500, balanced = TRUE)
```

```{r}
FeaturePlot(Locon4, slot = "scale.data", features = c("Phox2a", "Phox2b", "Eya1", "Eya2"), order = T)
```

```{r}
DimPlot(LoconTH4, reduction = "umap")
```
DimPlot(Locon4, reduction = "umap")
```{r}
FeaturePlot(LoconTH4, slot = "scale.data", features = c("Phox2a", "Phox2b", "Eya1", "Eya2"), order = T)
```
```{r}
FeaturePlot(LoconTH4, slot = "scale.data", features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Calca", "Hcrtr1", "Sstr2", "Calcr", "Slc6a2", "Slc18a2", "Dbh"), order = T)
```
```{r}
FeaturePlot(LoconTH4, slot = "scale.data", features = c("Dbh", "Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Calca", "Npy", "Hcrtr1", "Sstr2", "Calcr", "Galr1"), order = T)
```
```{r}
FeaturePlot(LoconTH4, features = c("Gal", "Grp", "Pdyn", "Cartpt", "Bdnf", "Calca", "Npy", "Hcrtr1", "Sstr2", "Calcr", "Galr1", "Dbh"), order = T)
```
```{r}
LC_markers <- c("Slc17a6", "Slc17a7", "Gad1", "Gad2", "Slc32a1", "Dbh", "Th", "Slc6a2", "Slc18a2", "Gal", "Grp", "Calca",
                "Cartpt", "Pdyn", "Bdnf", "Adcyap1", "Sst", "Npy", "Hcrtr1", "Sstr2", "Calcr", "Galr1", "Phox2a", "Phox2b",
                "Eya1", "Eya2")
DotPlot (Locon4, features = LC_markers)
                      
```
```{r}
Locon5 <- FindNeighbors(Locon, reduction = "pca", dims = 1:30, n.trees = 200, annoy.metric = "cosine", verbose = FALSE) %>%
    FindClusters(resolution = 2, algorithm = 1, verbose = FALSE) %>%
    RunUMAP( dims = 1:30, verbose = FALSE)
DimHeatmap(Locon5, dims = 1:20, cells = 500, balanced = TRUE)
```

```{r}
DimPlot(Locon5, reduction = "umap", label = TRUE)
```
```{r Markers neurons}
Locon5.markers <- FindAllMarkers(Locon5, only.pos = TRUE, test.use = "wilcox", min.pct = 0.1, logfc.threshold = 0.25)
Locon5.markers %>%
    group_by(cluster) %>%
    slice_max(n = 10, order_by = avg_log2FC)
```
```{r}
DotPlot (Locon5, features = LC_markers)
                      
```

```{r}
LoconTH4.markers <- FindAllMarkers(LoconTH4, only.pos = TRUE, test.use = "MAST", min.pct = 0.1, logfc.threshold = 0.25)
LoconTH4.markers %>%
    group_by(cluster) %>%
    slice_max(n = 10, order_by = avg_log2FC)
```

```{r}
LoconTH4.markers <- FindAllMarkers(LoconTH4, only.pos = TRUE, test.use = "LR")
LoconTH4.markers %>%
    group_by(cluster) %>%
    slice_max(n = 10, order_by = avg_log2FC)

LoconTH4.markers %>% filter(gene == "Grp")
```

```{r}
LoconTH4.markers <- FindAllMarkers(LoconTH4, only.pos = TRUE, test.use = "roc", min.pct = 0.1)
LoconTH4.markers %>%
    group_by(cluster) %>%
    slice_max(n = 10, order_by = avg_log2FC)
```
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